Epigenetic Research, Nucleosome Assembly, and Chromatin Remodeling Assay Development Tools
TarMart Solution Ecosystem & Related Targets
Comprehensive reagent toolkit for H4C15-centered epigenetic and chromatin biology research. Select your modality below:
| Component / Network | Product Description | Product Link |
|---|---|---|
| Antigen | H4C15 Recombinant Histone Protein Full-length native sequence, >95% purity by SDS-PAGE, Endotoxin <1EU/µg. Suitable for nucleosome reconstitution. |
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| Gene Delivery | H4C15 Lentivirus / Promise-ORF CMV-driven ORF for stable nuclear expression in mammalian cells; Puromycin selection marker included. |
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| Benchmark Ab | Anti-Histone H4 (Pan-H4 Clone) Rabbit recombinant mAb, ChIP-grade validated. Sequence verified constant regions. |
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| Validator | H4C15 siRNA Set (3 unique sequences) Chemically modified for nuclear stability; Include negative control. |
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| Related Target: H3C1 | Histone H3.1 (HIST1H3A) Core nucleosome component; required for octamer assembly assays |
View H3C1 Products |
| Related Target: SMARCA4 | BRG1 Chromatin Remodeler ATP-dependent nucleosome mobilization; functional partner for H4C15 dynamics |
View SMARCA4 Products |
Critical Assay Challenges & Technical Specifications
| Critical Assay Challenge | The TarMart Advantage (Technical Spec) |
|---|---|
| Native Nucleosome Assembly (Histone Octamer Formation) | HEK293-expressed H4C15 with correct folding; Theoretical MW verified by Mass Spec; High solubility in salt buffer for tetramer/octamer reconstitution |
| Histone Post-Translational Modification (PTM) Profiling | Unmodified basal state protein; Sequence verified to ensure no pre-existing acetylation/methylation artifacts; Controlled endotoxin to prevent cellular stress responses |
| Cross-species ChIP/ChIP-seq Validation | Human/Mouse/Rat H4C15 orthologs available (>98% sequence identity); Species-specific antibodies for comparative epigenetic studies |
| Intracellular Localization & Function | Lentivirus particles for stable integration; Codon-optimized ORF ensures high nuclear expression for complementation assays |
| Specificity Controls | Validated siRNA set included for knockdown confirmation; Isoform-specific antibody validation using recombinant proteins |
Live H4C15 Research Tracker
Market data changes daily. Access the latest global research status directly:
Global Research Landscape & Future Outlook
The study of replication-dependent histones, including H4C15, is experiencing renewed interest as researchers uncover the functional specialization of histone variants and mutations ("oncohistones") in cancer pathogenesis. While H4C15 itself encodes the canonical replication-dependent H4 protein, its precise role in nucleosome stability and chromatin accessibility makes it a critical reagent for drug discovery programs targeting chromatin remodelers (e.g., SMARCA2/4 inhibitors) and histone modification enzymes (HDACs, HATs, KMTs).
The current R&D focus has shifted from simple histone modification detection to dynamic nucleosome assembly assays and high-throughput screening for chromatin-modifying compounds. As the field moves toward synthetic lethality approaches involving chromatin regulators, demand for high-fidelity recombinant histones and cell-based assembly models continues to escalate.
Competitive Modality & Application Snapshot
Connect market trends to assay needs.
| Modality | Research Application | Key Players | Critical Assay Need |
|---|---|---|---|
| Biochemical Assays | Nucleosome assembly (histone chaperone-mediated) | Academic Core Facilities, Epitheran | High-purity recombinant H4C15 for octamer formation (Analytical SEC validation) |
| ChIP-seq / Epigenomics | Histone occupancy mapping | Active Motif, Abcam | ChIP-grade antibodies with validated specificity against H4C15 vs H4 variants |
| Structural Biology | Cryo-EM of nucleosome complexes | Structural Genomics Consortium | Homogeneous, aggregation-free H4C15 protein for complex formation |
| Cellular Complementation | Histone knockout rescue | Research Institutions | Lentivirus-delivered H4C15 ORF for stable cell line construction |