house mouse Ppp3ca/CN/CnA siRNA oligo Duplex (Gene ID: 19055) | TarMart for In Vitro RNAi and In Vivo Knock-Down Solutions

siRNAs are short, noncoding, double-stranded RNAs that leverage the RNA interference (RNAi) pathway to achieve highly specific gene silencing. They suppress gene expression by targeting and selectively degrading complementary messenger RNA (mRNA), through RNA-induced silencing complex (RISC).

You may order siRNAs through our website. The desired target can be searched by Symbol, Gene No, accession Number. Each query will yield three candidates. You may select the desired quantity at that point.

You may order siRNAs for the target gene of your choice by entering the gene name or Accession number at our website and selecting one of the listed siRNA candidates. You may also order custom designed siRNA if your model organism is not Human, Mouse or Rat. You can also order siRNAs by sequences, please email us.

For siRNAs, both the sense and antisense strands are synthesized at equimolar concentrations, then annealed and delivered in duplexed, lyophilized form. EPDC-treated water with every order. We recommend reconstituting to 100μM.

With a 2.5 nmol scale siRNA order, you can transfect ten 24-well plates at 20μM per transfection. For in vitro experiments, the standard small-scale purification method is OPC desalt. For in vivo experiments, HPLC purification is required. For in vivo experiments, HPLC purification is required.

To purify newly synthesized siRNA, we have several methods including OPC, HPLC and PAGE. We select a purification method depending on the end-use of the product:

• In vitro experiments, OPC purification is enough for use.
• In vivo experiments, HPLC is commonly used to reach the desired purity level. We use a reversed-phase resin for HPLC purification, and this will routinely yield up to 90% purity for 21-mer siRNA molecules.