domestic cat PPP3CA/ siRNA oligo Duplex (Gene ID: 101097144) | TarMart for In Vitro RNAi and In Vivo Knock-Down Solutions
Cat. No.: pGMoligo101097144
Leading Time:
3-7 working days
TarMart Lifescience offers domestic cat PPP3CA/ siRNA Oligo Duplex and Duplexes Package (Gene ID:101097144), designed for RNA interference (RNAi) in vitro and gene knock-down (KD) in vivo. Products undergo rigorous quality control for efficiency and specificity.
MSDS
🧬 GeneMedi siRNA Packages Selection 🧬
GeneMedi offers three siRNA packages for your selection:
In-vitro Grade-1 (Validated)
Includes one validated sequence.
Please note that sequence information will not be provided with this package.
In-vitro Grade Package
Includes three unvalidated sequences. If the validated option is unavailable, you may purchase this package.
Sequences will be provided with the shipment after payment is received.
Product Description
- In-vitro Grade-Package
- In-vivo Grade
| Catalog ID | pGMoligo101097144-sir-pack |
| Product Name | Pre-made domestic cat PPP3CA/ 3 unique siRNA oligo Duplexes Package |
| Product Type | siRNA oligo Duplexes package |
| Product Grade | in-vitro grade |
| Modification | Null |
| Validation | N/A |
| Size | 2.5nmol (about 1OD, 40-50µg) |
| Concentration/Formulation | 10µM in 250µl RNAse free DEPC water before lyophilization. |
| Purification | HPLC purified |
| Quality Control | Tested by ESI-MS |
| Sequences | Available with shipment |
| Components | 1.PPP3CA (domestic cat) siRNA oligo duplex -
2.5 nmol 2. Universal Scrambled Negative Control siRNA Duplex -2.5 nmol 3. Negative Control siRNA Duplex-Cy3 for Transfection Reorter -1 nmol 4. Positive Control Housekeeping gene siRNA Duplex-1nmol |
| Stability | One year from date of shipment when stored at -20°C. |
| Shipment Condition | RT in lyophilization |
| Catalog ID | pGMoligo101097144-sir-vivo |
| Product Name | Pre-made domestic cat PPP3CA/ siRNA oligo Duplex |
| Product Type | siRNA oligo Duplex |
| Product Grade | in-vivo grade |
| Modification | Phosphorothioate(PS) backbone modification+ Methoxy modification (2'-O-methyl)+ Fluorinated modification (2'-fluoro) |
| Validation | Validated |
| Size | 5mg (about 100OD, 250-300nm) |
| Concentration/Formulation | To be determined |
| Purification | HPLC purified |
| Quality Control | Tested by ESI-MS |
| Sequences | To be determined |
| Components | 1.PPP3CA (domestic cat) siRNA oligo duplex -
5mg(about 100OD, 250-300nm) 2. Universal Scrambled Negative Control siRNA Duplex 0.5mg Negative Control siRNA |
| Stability | One year from date of shipment when stored at -20°C. |
| Shipment Condition | RT in lyophilization |
Gene Information
| Gene Name | PPP3CA |
| Gene ID | 101097144 |
| Gene Alias/Synonyms | |
| mRNA isoform Accession Number/Ref seq | |
| Species | domestic cat |
| UniPro ID |
FAQ
What is siRNA and how does it work?
siRNAs are short, noncoding, double-stranded RNAs that leverage the RNA interference (RNAi) pathway to achieve highly specific gene silencing. They suppress gene expression by targeting and selectively degrading complementary messenger RNA (mRNA), through RNA-induced silencing complex (RISC).
How do I order siRNAs?
You may order siRNAs through our website. The desired target can be searched by Symbol, Gene No, accession Number. Each query will yield three candidates. You may select the desired quantity at that point.
Can I order siRNAs targeted for a specific gene?
You may order siRNAs for the target gene of your choice by entering the gene name or Accession number at our website and selecting one of the listed siRNA candidates. You may also order custom designed siRNA if your model organism is not Human, Mouse or Rat. You can also order siRNAs by sequences, please email us.
What form will my order be in?
For siRNAs, both the sense and antisense strands are synthesized at equimolar concentrations, then annealed and delivered in duplexed, lyophilized form. EPDC-treated water with every order. We recommend reconstituting to 100μM.
I want to conduct an in vitro experiment. What scale should I choose? What purification should I select?
With a 2.5 nmol scale siRNA order, you can transfect ten 24-well plates at 20μM per transfection. For in vitro experiments, the standard small-scale purification method is OPC desalt. For in vivo experiments, HPLC purification is required. For in vivo experiments, HPLC purification is required.
I would like to know how the synthesized siRNA is purified.
To purify newly synthesized siRNA, we have several methods including OPC, HPLC and PAGE. We select a purification method depending on the end-use of the product:
- In vitro experiments, OPC purification is enough for use.
- In vivo experiments, HPLC is commonly used to reach the desired purity level. We use a reversed-phase resin for HPLC purification, and this will routinely yield up to 90% purity for 21-mer siRNA molecules.