Recombinant multi-species PLEC/Plectin protein for cell curture, in vitro study, in vivo study, benchmark, drug discovery & MOA research and positive control for the diagnosis

Genemedi produces recombinant Human, Cynomolgus/ Rhesus macaque, Rat, Mouse, Feline, Canine, Bovine, Equine PLEC/Plectin protein for cell curture, in vitro study, in vivo study, benchmark, drug discovery & MOA research and positive control for the diagnosis.
Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (reviewed in PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used. [provided by RefSeq, Aug 2011]
The Alternative Names of target: EBS1,EBS5A,EBS5B,EBS5C,EBS5D,EBSMD,EBSND,EBSO,EBSOG,EBSPA,HD1,Hemidesmosomal protein 1 (HD1),LGMD2Q,LGMDR17,PCN,PLEC,PLEC1,PLEC1b,PLTN,Pcn,Plec,Plec1,Plectin,Plectin-1,Pltn

Target products collectionGo to PLEC/Plectin products collection >>
(antibodies, antigen, VLP, mRNA, ORF viral vector, etc)

pdfMSDSpdfCOApdfDatasheet

Product information

Catalog No. Product Name Species Reactivity
GM-Tg-hg-IP9632-Ag-1/ GM-Tg-hg-IP9632-Ag-2 Human PLEC/Plectin protein Human
GM-Tg-rg-IP9632-Ag-1/ GM-Tg-rg-IP9632-Ag-2 Rat PLEC/Plectin protein Rat
GM-Tg-mg-IP9632-Ag-1/ GM-Tg-mg-IP9632-Ag-2 Mouse PLEC/Plectin protein Mouse
GM-Tg-cynog-IP9632-Ag-1/ GM-Tg-cynog-IP9632-Ag-2 Cynomolgus/Rhesus macaque PLEC/Plectin protein Cynomolgus/ Rhesus macaque
GM-Tg-felg-IP9632-Ag-1/ GM-Tg-felg-IP9632-Ag-2 Feline PLEC/Plectin protein Feline
GM-Tg-cang-IP9632-Ag-1/ GM-Tg-cang-IP9632-Ag-2 Canine PLEC/Plectin protein Canine
GM-Tg-bovg-IP9632-Ag-1/ GM-Tg-bovg-IP9632-Ag-2 Bovine PLEC/Plectin protein Bovine
GM-Tg-equg-IP9632-Ag-1/ GM-Tg-equg-IP9632-Ag-2 Equine PLEC/Plectin protein Equine

Size： 1mg | 10mg | 100mg



Product Description

Catalog No. GM-Tg-hg-IP9632-Ag-1/ GM-Tg-hg-IP9632-Ag-2; GM-Tg-rg-IP9632-Ag-1/ GM-Tg-rg-IP9632-Ag-2;
GM-Tg-mg-IP9632-Ag-1/ GM-Tg-mg-IP9632-Ag-2; GM-Tg-cynog-IP9632-Ag-1/ GM-Tg-cynog-IP9632-Ag-2;
GM-Tg-felg-IP9632-Ag-1/ GM-Tg-felg-IP9632-Ag-2; GM-Tg-cang-IP9632-Ag-1/ GM-Tg-cang-IP9632-Ag-2;
GM-Tg-bovg-IP9632-Ag-1/ GM-Tg-bovg-IP9632-Ag-2; GM-Tg-equg-IP9632-Ag-1/ GM-Tg-equg-IP9632-Ag-2
Products Name PLEC/Plectin protein
Species Human, Cynomolgus/ Rhesus macaque, rat, mouse, Feline, Canine, Bovine, Equine
Target Name PLEC/Plectin
Protein Sub-location Introcelluar Protein
Isotypes Recombinant protein
Expression platform Mammalian cell
Bioactivity validation Affintiy&bioactivity validated by ELISA, cell culture validated.
Tag His
Products description Recombinant Human, Cynomolgus/ Rhesus macaque, Rat, Mouse, Feline, Canine, Bovine, Equine PLEC/Plectin protein was expressed in mammalian cell expression system and is expressed with 6 HIS tag at the C-terminus for cell culture, ELISA or other affinity binding assay or functional assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic).
Purity Purity: ≥95% (SDS-PAGE)
Application In vitro study, in vivo study, benchmark, positive control for the diagnosis. Biological drug disovery including cell culture, assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic) and mechanism of action (MOA) research.
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4);
For PSB2, reconstituted with 0.9% sodium chloride;
For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference






    About Gmab

    GDU
    GDU

    GMab, developed by GeneMedi, constitutes an advanced library of recombinant monoclonal antibodies, each meticulously designed to target specific molecular entities. Leveraging the sophisticated capabilities of GM’s Taurus™ and LIBRA™ platforms, GMab synthesizes antibodies characterized by high binding affinity, exceptional physicochemical stability, and optimal developability profiles.
    Through expression in mammalian cell lines, GMab has been established as a paradigmatic reference antibody. It holds significance in myriad domains of biological drug discovery, encompassing cellular cultivation, innovative assay methodologies, strategic animal model systematization, in-depth pharmacokinetic & pharmacodynamic (PK/PD) modeling, and intricate mechanism of action (MOA) investigations.